Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: The construction and the antitumor mechanism of DHPL. (A) The construction procedure of DHPL. (B) The antitumor and ultrasound imaging mechanism of DHPL.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Imaging

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Characterization of DHPL. (A) UV-vis absorption spectrum of DHPL. (B) Representative transmission electron microscope (TEM) images of HAuNS, OMP-HAuNS and DHPL, respectively. (C) The temperature elevation curves of DHPL-59 containing various HAuNS concentrations or PBS under NIR laser (1 W/cm 2 ). (D) SA-DOX release from DPL or DHPL-59 with (2 W/cm 2 for 3 min, at 2 and 6 h) or without NIR laser.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Transmission Assay, Microscopy

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Temperature-induced size change of DHPL. (A) Particle size changes of DHPL-29 and DHPL-59 in PBS at various temperatures (n=3). (B) Particle size changes of DHPL-29 and DHPL-59 in HPMC solutions at various temperatures (n=3). (C) Particle size changes of DHPL-59 in PBS during three heating-cooling cycles at four different temperatures (n=3). (D) Particle size changes of DHPL-29 in PBS during three heating-cooling cycles at four different temperatures (n=3). (E) The particle size changes of DHPL-59 and DHPL-29 in HPMC solutions during three heating-cooling cycles at 82 °C (n=3). (F) The photomicrographs (left) and fluorescence photomicrographs (right) of the microbubbles produced by DHPL-59 after NIR light irradiation (2 W/cm 2 , 2 min). Scale bars, 20 μm.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Fluorescence, Produced, Irradiation

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Ultrsound imaging in vitro . (A) A hole model (pore size: 1 cm) made by 1 % Sephadex gel as the imaging matrix with an 808 nm laser fiber inserted. (B) Representative ultrasound images of HAuNS@DPL (0.5 mg/mL HAuNS) under NIR light irradiation (1 W/cm 2 ). (C) Representative ultrasound images of DHPL-59 (0.5 mg/mL HAuNS) under NIR light irradiation (1 W/cm 2 or 2 W/cm 2 ). (D) Representative ultrasound images of PBS as a negative control after NIR light irradiation (2 W/cm 2 ). Yellow circles: region of interest (ROI). Scale bars, 0.5 cm.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Imaging, In Vitro, Pore Size, Irradiation, Negative Control

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Cellular internalization. Cellular uptake of DPL (A) , DHPL (B) , SA-DOX (C) by MCF-7 cells in 2, 6, 12 h observed using confocal microscopy. Cellular uptake of DPL (D) , DHPL (E) , SA-DOX (F) by 4T1 cells in 2, 6, 12 h observed using confocal microscopy. Scale bars, 50 μm or 20 μm (x3 magnified images). Quantitative analysis of DOX fluorescence intensity in MCF-7 (G) or 4T1 (H) cells.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Confocal Microscopy, Fluorescence

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Cytotoxicity. Cell viability of MCF-7 (A) and 4T1 (B) cells treated with SA-DOX, DOX·HCl, DPL, HPL, and DHPL followed by NIR light irradiation (1 W/cm 2 , 3 min). Cells without laser irradiation were used as controls.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Irradiation

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Ultrasound imaging in vivo . In vivo ultrasound images of tumor sites in 4T1 tumor-bearing mice at 24 h (A) or 48 h (B) post-injection of DHPL-29. The tumor sites were treated with NIR laser irradiation (2 W/cm 2 ) for 2 min or 5 min. (C) In vivo ultrasound images of tumor sites in 4T1 tumor-bearing mice before and during the perfusion of SonoVue ® . The yellow dotted line indicates tumor sites. The yellow arrow indicates microbubbles produced after NIR light irradiation. Scale bars, 0.5 cm.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: Imaging, In Vivo, Injection, Irradiation, Produced

Journal: Theranostics

Article Title: Temperature-controlled, phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light

doi: 10.7150/thno.23885

Figure Lengend Snippet: Biodistribution and antitumor effect in vivo . (A) Representative in vivo fluorescence imaging of the 4T1 tumor-bearing mice at different time point after i.v. injection of DiR-labeled DHPL. The black circles indicate tumors. Ex: 748 nm; Em: 780 nm. (B) The tumor growth curves for 4T1 tumor-bearing mice treated with PBS (n=7), DOX·HCl (n=7), DPL (n=7), HPL plus NIR light irradiation (1 W/cm 2 , 3 min, n=7) and DHPL plus NIR light irradiation (1 W/cm 2 , 3 min, n=7). The blue arrow indicates injections and the red arrow indicates laser irradiations. (C) Representative photographs of tumors in all groups at days 0, 12, and 20. (D) Photographs of tumors in all the groups at day 22. (E) Average tumor weights in different treatment groups. Tumors were removed on day 22. (F) The photomicrographs of H&E-stained (left), tunnel-stained (middle) and Ki-67-stained (right) tumor slides after different treatments. Scale bars, 100 μm.

Article Snippet: When 4T1 tumor volume reached about 300 mm 3 , the mice were injected with 0.2 mL DiR-labeled DHPL (DOX: 0.5 mg/mL) through tail vein and observed by an in vivo imaging system (Maestro EX, CRI Inc., Woburn, MA) at 24, 48, 72 h post-injection.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Labeling, Irradiation, Staining